WHOLISTIC imaging – sagittal view.
Sagittal view of Tg(ubi:tTA; TRE:GCaMP7f) line with major organs indicated, imaged with 20× 0.75 NA air objective (sped up ~50×).
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WHOLISTIC imaging – dorsal view.
Dorsal view of Tg(ubi:tTA; TRE:GCaMP7f) line with major organs indicated, imaged with 10× 0.45 NA air objective (sped up ~50×).
WHOLISTIC imaging of enteric activity highlighting visceral motion.
Sagittal view of Tg(ubi:tTA; TRE:GCaMP7f) line with major organs indicated, imaged with 40× 1.15 NA water objective (sped up ~50×).
WHOLISTIC imaging of cold response.
Dorsal view of Tg(ubi:tTA; TRE:GCaMP7f) line, imaged with 40× 1.15 NA WI objective (sped up ~50×) in response to cold water delivery (onset indicated on the top right).
Registration results.
Sagittal view of the registration channel derived from Tg(β-actin:mCherry-CAAX) line, imaged with 20× 0.75 NA air objective. Left: data pre-registration; Right: data post-registration.
Whole-Body Expansion Microscopy.
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Visualization of a transgenic animal labeling the autonomic nervous system (Tg(phox2bb:eGFP)), stained against eGFP (magenta) with total protein stain (Alexa488-NHS, green) (WB-ExM-IF, expanded ~2×).
Visualization of hindbrain ependymal sheet using Whole-Body Expansion Microscopy with immunofluorescence.
Sagittal view of a transgenic sample labeling the ventricular system (Tg(foxj1a:eGFP)), stained against eGFP (magenta) using WB-ExM-IF (expanded ~2×), highlighting the ventral ependymal sheath.
3D geometric model of young zebrafish.
3D model derived from Whole-Body Expansion Microscopy data containing the major organs of a young zebrafish, built and rendered using Blender.
Hypoxia induced blood flow redirection.
Sagittal section of a transgenic animal with fluorescently labeled red blood cells (Tg(gata1:dsRED)), imaged with 20× 0.75 NA air objective (sped up ~1.5×), showing blood flow during normoxia (left) and hypoxia (right), with a notable reduction in mesenteric blood flow, especially in the endocrine pancreas region.