PhotoMap: unbiased mapping of Expansion Microscopy deformation fields

Overview

To quantify deformation introduced by expansion in a less biased manner, we developed PhotoMap, a method that measures the gel’s deformation field from a pre-imposed reference pattern. A regular grid is photobleached into the sample prior to expansion and re-imaged afterwards, enabling straightforward visualization and quantification of spatial distortions.

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Also available on protocols.io: dx.doi.org/10.17504/protocols.io.dm6gp9wxjvzp/v4

Citation

Virginie M S Ruetten, Amy Hu, Misha B. Ahrens, Paul Tillberg. PhotoMap: unbiased mapping of Expansion Microscopy deformation fields.

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